Prof. Vasco Bonifácio (DBE, iBB) and collaborators from FCT-NOVA University of Lisbon report in Materials Today Chemistry a green protocol for synthesising dual-templating molecularly imprinted polymers (D-MIPs) in supercritical CO₂. Designed to target both lysine and leucine, these D-MIPs achieve imprinting factors from 1.3 to 3.8 over non-imprinted polymers (NIPs). Static binding assays confirm superior capacity and selectivity of the D-MIP versus a single-template MIP (O-MIP), revealing significant dynamic binding capacity, even in more complex media (such as selectivity for their template–molecules in different biomolecules solutions). The findings highlight the ability of the D–MIP to selectively recognize its template molecules even in the presence of a protein, showcasing its potential for real–world applications in biopurification. Both MIPs were found to be attractive, low–cost, and sustainable solutions for obtaining tailor–made biorecognition materials, emphasizing their applicability as stationary phases in chromatographic techniques for biopurification processes within the biotechnology and biopharmaceutical industries. See more.